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Essential genes include housekeeping genes (critical for basic cell functions) [135] as well as genes that are expressed at different times in the organisms development or life cycle. [136] Housekeeping genes are used as experimental controls when analysing gene expression, since they are constitutively expressed at a relatively constant level.
FISSEQ Single cell transcriptome sequencing in situ, i.e. without dissociating the cells. Oscope: a statistical pipeline for identifying oscillatory genes in unsynchronized single cell RNA-seq experiments. SCUBA [116] Extracting lineage relationships and modeling dynamic changes associated with multi-lineage cell differentiation.
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